I am loading cellranger data into seuratobject as following, but it keeps showing Error in "Barcode file missing. Expecting barcodes.tsv.gz" the running code: > #clean up your environment > rm(list=ls()) > # Load required libraries > library(Seurat) > library(dplyr) > # Load required library for reading Matrix Market files > library(Matrix) > # For output from CellRanger >= 3.0 with multiple data types > data_dir <- 'C:/Users/Administrator/Desktop/10x_raw' > list.files(data_dir) # Should show barcodes.tsv.gz, features.tsv.gz, and matrix.mtx.gz [1] "GSM7494257_AML16_DX_raw_barcodes.tsv.gz" "GSM7494257_AML16_DX_raw_genes.tsv.gz" [3] "GSM7494257_AML16_DX_raw_matrix.mtx.gz" > data <- Read10X(data.dir = data_dir) Error in Read10X(data.dir = data_dir) : Barcode file missing. Expecting barcodes.tsv.gz > seurat_object = CreateSeuratObject(counts = data$`Gene Expression`) Error in data$`Gene Expression` : object of type 'closure' is not subsettable I am sure this file inside the directory. Dose anyone know what's the issue? Thanks in advance.