I am working with an amplicon kit from Swift Bioscience and they provide the primer locations in what they call a "masterfile". The format is like this: $ head -n2 Acel-Amplicon_56g_masterfile.txt 1 43814951 43815064 MPL_S505N-A519T_F/2 43814932 43814951 MPL_S505N-A519T_F/2_primer 43815064 43815081 MPL_S505N-A519T_R/1_primer GCCTGGATCTCCTTGGTGA CTTCGGCTCCACCTGGT 1 115256480 115256576 NRAS_G60-S65_new_R/1 115256457 115256480 NRAS_G60-S65_new_R/1_primer 115256576 115256602 NRAS_G60-S65_new_F/2_primer TGATGGCAAATACACAGAGGAAG CAGGATTCTTACAGAAAACAAGTGGT This is the format which is understood by [Swift's primer clipping tool][1], but in order to use other tools, I would like to convert it to BEDPE. However, I can't do that since I am not sure what the fields in this file are. This is what I think thy are: 1. Chromosome 2. Target region start 3. Target region end 4. 5' Primer name 5. 5' Primer start 6. 5' Primer end 7. 3' Primer name 8. 3' Primer start 9. 3' Primer end 10. 5' Primer sequence 11. 3' primer sequence Is my interpretation correct? Also, is this a standard file format or something specific to the Swift tool? I couldn't find any documentation about a "masterfile" format online. [1]: https://github.com/swiftbiosciences/primerclip