I have normalized expression values of some genes in 76 patients and the how many days they have survived from their diagnosis with cancer like below Survival days MTHFD1 SH3PXD2A CAV1 ACTB COL5A2 CDKN2C BAG3 RALA DST STEAP1 FZD1 IGFBP2 RBP1 GSM482796 1450 -0.538 0.261 0.368 0.671 0.194 -0.43 -0.179 0.551 -0.117 0.18 -0.527 -0.703 -0.0586 GSM482797 936 -0.209 0.097 -0.9 -0.307 0.0873 -0.516 -0.211 0.089 -0.0729 -0.0546 -0.132 -0.481 0.0215 GSM482798 936 -0.487 0.0853 -0.518 0.162 0.0326 -0.264 -0.0957 0.237 -0.0855 -0.243 -0.137 0.0581 0.11 GSM482799 2060 -0.355 0.0949 -0.198 0.232 0.0123 -0.506 0.0424 0.0626 0.128 0.0602 -0.538 -0.0969 0.277 GSM482800 2574 -0.547 0.0836 -0.81 -0.211 0.0928 -0.536 -0.301 0.126 -0.327 0.129 -0.251 0.536 0.02 GSM482801 2313 -0.484 -0.333 -1.13 0.312 -0.0508 -0.848 -0.0893 0.164 -0.15 -0.2 -0.85 0.0732 0.12 GSM482802 176 -0.343 0.194 1.29 0.436 0.221 -0.717 0.0727 0.65 1.29 0.857 0.00985 -0.384 0.289 GSM482803 NA -0.623 0.176 -0.581 0.341 -0.0391 -0.446 -0.00736 -0.0541 -0.157 -0.197 -0.349 0.304 0.26 GSM482804 1327 -0.381 0.0195 -0.939 0.024 0.0151 -0.513 0.832 -0.055 0.0195 0.431 -0.673 -0.521 0.0575 GSM482805 1575 -0.167 0.0854 -0.411 0.0854 0.105 -0.296 0.101 0.31 0.798 -0.12 -0.152 0.119 0.142 GSM482806 687 -0.439 -0.0619 -1.06 0.0728 0.0771 -0.155 -0.232 0.164 -0.0181 0.196 0.449 0.748 -0.0259 GSM482807 736 -0.744 -0.191 -1.37 0.148 0.0684 -0.698 -0.292 0.187 -0.0628 -0.396 -0.598 -0.488 0.0977 GSM482808 816 -0.607 -0.194 -0.358 0.0134 0.159 -0.26 0.0238 -0.374 0.555 0.274 0.358 -0.0953 0.171 GSM482809 253 -0.354 0.121 -1.22 0.402 -0.0781 -0.699 -0.159 0.345 -0.0888 0.0523 -0.888 -0.759 0.0681 GSM482810 477 -0.607 -0.201 -1.05 0.551 -0.0554 -0.655 -0.148 -0.0741 -0.78 -0.31 -0.716 -0.154 0.0134 GSM482811 1035 -0.516 0.346 -0.62 0.0466 0.0884 -0.244 0.107 0.101 0.807 0.473 0.291 -0.417 0.0251 GSM482812 1984 -0.281 -0.0653 -0.917 0.255 -0.0167 -0.683 -0.0696 0.559 -0.175 -0.253 -0.458 -0.536 0.0461 GSM482813 2045 -0.42 0.0355 -0.792 0.513 0.158 -0.245 0.514 0.184 -0.211 -0.364 -0.573 0.183 0.049 > ***EDITED*** I have taken mean of the expression values for each patient and I have how many days each patient survived since diagnosis as below > head(km) samples time gene 1 GSM482796 1450 -0.11931980 2 GSM482797 936 -0.12209097 3 GSM482798 936 -0.10390257 4 GSM482799 2060 -0.07646939 5 GSM482800 2574 -0.16656837 6 GSM482801 2313 -0.12507939 > I done so but nothing I got > fit <- survfit(Surv(time) ~ gene, data = km) > print(fit) Call: survfit(formula = Surv(time) ~ gene, data = km) n events median 0.95LCL 0.95UCL gene=-0.333140816 1 1 859 NA NA gene=-0.307846735 1 1 347 NA NA gene=-0.303559694 1 1 1339 NA NA gene=-0.290518776 1 1 61 NA NA The question is if these genes increase survival time or not but likely I am doing wrong Any help please? Thanks