*This question has also been asked on [Biostars](https://www.biostars.org/p/9488897/)* I ran Kallisto with the --pseudobam option. How do I extract all the short reads that are mapped to a single transcript (e.g. ENST00000367969.8)? As a person without any previous SAM/BAM experience, I tried the following things without success. Really appreciate some help here. I first tried: samtools view -S pseudoalignments.bam ENST00000367969.8 but got the following error: ``` [E::idx_find_and_load] Could not retrieve index file for 'pseudoalignments.bam' [main_samview] random alignment retrieval only works for indexed BAM or CRAM files. ``` I tried to index the file using: samtools index pseudoalignments.bam but get the following error: ``` [E::hts_idx_push] NO_COOR reads not in a single block at the end 79729 -1 [E::sam_index] Read 'A00742:184:HLTF2DSXY:4:1101:4417:1000' with ref_name='ENST00000556431.1', ref_length=4142, flags=83, pos=2098 cannot be indexed samtools index: failed to create index for "pseudoalignments.bam" ```