I have multiple SRR files to download, and their accession is in a list: SRR9004325 SRR9004326 SRR9004327 I read the [NCBI document][1] but don't find how to achieve this using the command "fastq-dump". [1]: https://www.ncbi.nlm.nih.gov/sra/docs/sradownload/ And I noticed that if I use the command `prefetch`, I can download from the list file: prefetch --option-file SraAccList.txt So is there any parameter in "fastq-dump" to download accessions stored in a file? As @terdon said, it is better to use `fasterq-dump`, and the command: `fasterq-dump --option-file SraAccList.txt` doesn't work.