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Help with DIA-Mass Spectrometry data analysis with several conditions (limma?)
I read something about Limma package, but not sure if it can be used in this DIA-ms data and neither sure of how to use limma for it (I already check the vignette with no succeed)
UPDATE: I tried to use … Limma as follows:
fit1 <- lmFit(norm.data_collapsed, design)
fit2 <- contrasts.fit(fit1, contrasts = contrast)
fit3 <- eBayes(fit2)
limma.res.01 <- topTable(fit3, coef = comb.contrast[1], n= Inf)
This …