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use for general applications of high-throughput nucleotide sequencing methods that use short-read technology (e.g. Illumina, IonTorrent). For long-read sequencing, use 'long-read', or a more specific tag if applicable (e.g. nanopore, pacbio).
7
votes
QC measures for NGS sequencing
We routinely run both FastQC and FastQ Screen on all of our raw sequencing reads. FastQ Screen is a tool for detecting cross-species contamination. MGA is another similar tool.
There are then lots of …
2
votes
Importance of Proper Pairs vs Aligned Reads for RNASeq data
If you check the RSeQC docs you can get an explanation of how the inner distance stats work. By the look of your inner-distance MultiQC plot I guess that your sequencing is 2x100bp reads. This plot sh …