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To cut from the end. Trimming is often done on raw Illumina reads to discard low-quality bases or adapter sequences. Questions related to trimming algorithms, when, how to trim, trimming software.

4 votes

How can I systematically detect unknown barcode/adapter sequences within a set of samples?

You mention that FastQC "fails to find the actual adapter sequences" - I guess you mean in the Adapter Sequence Contamination plot. However, the kmer and Sequence Content Plots are often useful even w …
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