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Questions specific to interacting with and post-processing sequence alignments using the SAMtools package. For questions specifically about formats SAM, BAM or CRAM use tags sam or bam,

8 votes

How to subset a BAM by a list of QNAMEs?

Using samtools 1.12 or greater: samtools view -N qnames_list.txt -o filtered_output.bam input.bam OLD ANSWER: When it comes to filter by a list, this is my favourite (much faster than grep): # given a … bam file "aln.bam" and a list of read names "reads.txt": samtools view -h aln.bam \ | awk 'FNR==NR {reads[$1];next} /^@/||($1 in reads)' reads.txt - \ | samtools view -b - > filtered_aln.bam Basically …
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