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2 votes
1 answer
128 views

nextflow: merge lane files from samples forward and reverse read

I'd like to merge files from different lanes for forward and reverse reads. For e.g., if a sample A has three lanes in forward, and three reverse, ultimately one reverse and one forward is to be gener …
Death Metal's user avatar
1 vote
1 answer
128 views

nextflow: unable to find index

I've a pipeline with many processes. One of the steps is to run lancet tool. Lancet tool runs per chromosome (1-22, x,y). The input in the lancet step is output from the applybqsr (gatk). There are tu …
Death Metal's user avatar
1 vote
1 answer
213 views

Nextflow - Missing value declared as output parameter - output variable defined in script

fastq.gz tiny_tumor_R2.fastq.gz tiny_normal_R1.fastq.gz tiny_normal_R2.fastq.gz The expected output from the mutect2 is tiny.mutect2out.vcf that is ${sample_name}".mutect2out.vcf" I split tiny_normal using nextflow
Death Metal's user avatar
4 votes
1 answer
160 views

nextflow: tumor normal sample - how to make code organic

I'm developing pipeline in nextflow it uses tools: fastp, bwa mem index, bwamem, gatk mark duplicates, gatk setupnmd, gatk applybqsr, gatk recalibrate. …
Death Metal's user avatar
2 votes
0 answers
134 views

Nextflow: pass reads variable to process in another script

This question has been asked and answered on Stack Overflow I'm new to nextflow, channel, process. Kindly bear with me. I'd like to create a pipeline, pass paired reads from one to another process. …
Death Metal's user avatar