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Doubt about using TPM for statistics

My PI say to make the statistics over the ration response (expression_treatment/expression_control - 1) and my question is the following: Can I use TPM to calculate this ratio and make following statistics … I read in the documentation of DESeq2 that TPM we can't use this normalisation method to differential expression and my my other question is Why shouldn't? …
Manuel Sánchez Mendoza's user avatar