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Reads are the sequences output by a sequencing machine after the raw signal (e.g. light, electricity) is converted into bases by a basecaller.

2
votes
For short reads, the typical and the most widely used solution is to correct away sequencing errors before assembly. You can correct errors with k-mer spectrum, a trie or multi-alignment. …
answered Oct 22 '17 by user172818
6
votes
The following javascript does what you want. You need node.js to run it. It should be easy to translate the code to Python. I have not carefully tested it. Use with caution. EDIT (response to new com …
answered Mar 14 '18 by user172818
6
votes
I am commenting on this part: The algorithm only removes low quality bases from the end until it reaches a good quality base. If there is a bad quality base beyond that, it is not trimmed. Accor …
answered Feb 26 '18 by user172818