2 votes
Accepted

Some definitions about RNA-seq

I am pretty sure that is read length. Both reads in a pair (2x) can be 50, 75, ... 250 bases long. And I am even more sure you will find this information on the webpage of the provider and if not, you ...
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  • 5,327
1 vote

Low custom tdtomato gene content

We had the same issue. Turned out, our tdTomato transgene was followed up by WPRE and BGH polyA signals. These sequences are quite large, and their presence precludes sequencing into the tdTomato ...
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