3 votes
Accepted

Basecalling process carried out by full_1dsq_basecaller.py/.exe

My understanding is that the 1D$^2$ process is carried out in three stages: Carry out a 1D calling of reads (i.e. what the standard Albacore does at the moment) Link reads that are similar to each ...
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1 vote

Help with 1D^2 library shearing

Short template DNA is a big problem with nanopore sequencing; it doesn't easily show up on a gel if it's well distributed across different lengths (as appears to be the case here from the read length ...
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1 vote
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Removing repeated reads from nanopore 1D² reads

You can exclude the 1D² reads by generate a list of the ids from the 1D² reads, then filtering those reads out from the 1D fastq files. Here's one way to do that using some accessory scripts that I ...
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