3
votes
Accepted
Basecalling process carried out by full_1dsq_basecaller.py/.exe
My understanding is that the 1D$^2$ process is carried out in three stages:
Carry out a 1D calling of reads (i.e. what the standard Albacore does at the moment)
Link reads that are similar to each ...
- 12.8k
1
vote
Help with 1D^2 library shearing
Short template DNA is a big problem with nanopore sequencing; it doesn't easily show up on a gel if it's well distributed across different lengths (as appears to be the case here from the read length ...
- 12.8k
1
vote
Accepted
Removing repeated reads from nanopore 1D² reads
You can exclude the 1D² reads by generate a list of the ids from the 1D² reads, then filtering those reads out from the 1D fastq files. Here's one way to do that using some accessory scripts that I ...
- 12.8k
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