Hot answers tagged

1

The core idea behind BUSCO is that there are a number of genes that are Unique Single-Copy Orthologues (USCOs) that should be present in most genomes and should only appear once in the genome. This is only a guess, however, and not a universal rule. Sometimes genomes can have multiple copies of these USCOs, and these duplicated genes reduce the overall score ...


1

It's hard to say for sure without seeing the exact commands you used. However, Orthofinder identifies/defines single-copy orthologues as those genes present in a single copy in all species being compared in the analysis. If a BUSCO orthologue is present in more than one copy in one of the species being compared (could be multi-copy, could be fragmented etc.),...


Only top voted, non community-wiki answers of a minimum length are eligible