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2 votes

Running cellranger on scRNASeq data with feature barcoding (10x + antibody capture)

Check your FASTQ headers - if they've been demultiplexed, the index barcodes can be found in the header. The barcodes are usually separated by a + in the comment ...
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2 votes

In cellranger, can I change the minimum number of reads for autodetecting chemistry?

I don't think you can reduce the number of reads required for autodetection, and it would be a bad idea anyway, because the guess could go wrong. From 10X Q&A: TotalSeq B is specifically designed ...

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