New answers tagged ensembl
2
votes
How to identify which isoforms of a gene are actually expressed in my data?
If you do as @james-hawley rightly suggests, keep in mind that pseudo-alignment could detect a lot of transcripts with low TPM that are actually false positives, as reported here (it's a paper ...
- 96
4
votes
How to identify which isoforms of a gene are actually expressed in my data?
Tools like kallisto or salmon can perform a process call pseudo-alignment that matches reads from RNA-seq data to transcript isoforms present in a reference genome.
You can then look at the output of ...
- 1,192
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