New answers tagged

2 votes

How to identify which isoforms of a gene are actually expressed in my data?

If you do as @james-hawley rightly suggests, keep in mind that pseudo-alignment could detect a lot of transcripts with low TPM that are actually false positives, as reported here (it's a paper ...
user avatar
  • 96
4 votes

How to identify which isoforms of a gene are actually expressed in my data?

Tools like kallisto or salmon can perform a process call pseudo-alignment that matches reads from RNA-seq data to transcript isoforms present in a reference genome. You can then look at the output of ...
user avatar
  • 1,192

Top 50 recent answers are included