4 votes
Accepted

Obtain CD (cluster of differentiation) marker expression information

I can offer this as a way to scrape the data from the Abcam resource: ...
neilfws's user avatar
  • 646
3 votes
Accepted

What are negative FSC-A values in flow cytometry data?

This is probably a matter of calibration. If this is not the raw data (actual voltage curves), look at that. As an area below 0 is not possible, it has to be a calibration issue, either in the ...
bukwyrm's user avatar
  • 146
2 votes

Finding peaks and estimating cell population sizes in multi-dimensional flow cytometry data

Automated gating methods are gaining popularity over manual and time consuming analysis in say FlowJo, you should take a look at the openCyto bioconductor package this is a framework which builds on ...
Matt Bashton's user avatar
  • 1,069
2 votes

Finding peaks and estimating cell population sizes in multi-dimensional flow cytometry data

There are quite a few algorithms developed for the automatic classification of multidimensional flow cytometry data, you can see a (not so recent?) review here: http://www.nature.com/nmeth/journal/v10/...
Leo Martins's user avatar
1 vote

FlowSOM multi-step clustering

The two step approach will be used in any cluster algorithm (except see "network theory" below), you just may not see it. Clustering methods are very common in genomics, they are popular ...
M__'s user avatar
  • 12.1k
1 vote

What is the correct way of dealing with the analysis of data from flow cytometry?

If your goal is to simply "to quantify the change in the amount of molecule," then you can do that at the population-average level by simply taking the average antibody signal for the ...
David Ross's user avatar

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