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The longest possible transcriptome contig reflects the longest possible transcript. In the human genome, that's possibly Titin, which is ~35K aa (that's ~105Kb) and will be longer pre-splicing. The longest possible genome contig reflects the longest chromosome. In the human genome that's chr1 at ~250Mb. In practise your contigs/scaffolds will be a lot ...


2

This is quite a similar question to this one, but I notice that you're specifically asking about a probability distribution rather than differential expression. That sounds like gene dispersion and/or shot noise; a good reference for me on that has been the documentation for DESeq2: http://www.bioconductor.org/packages/release/bioc/vignettes/DESeq2/inst/doc/...


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