3
votes
paired-end short reads: will one file suffice?
I suspect you won't be able to avoid assembling or aligning. If you are working with strains that don't have their own, well studied reference genome and so want to avoid aligning so as not to bias ...
- 9,422
1
vote
paired-end short reads: will one file suffice?
The problem with using single-end short reads is that they're often not long enough to uniquely match to a database (especially for highly-conserved genes, like the ribosomal RNAs). Whether or not ...
- 13.5k
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