6
votes
Accepted
Can I stop my nanopore sequencing run if there are no more reads being produced?
Yes, you can click "stop acquisition" and your run won't be negatively affected. All of the reads are saved as they are generated. I am not sure how this will impact live basecalling though if that is ...
- 3,141
6
votes
Accepted
How does MinKNOW classify 1D reads as "pass" or "fail"?
The classification is indeed based purely on comparing a mean to a threshold... but it's not the mean of the Phred scores that represent base quality on a logarithmic scale, but rather the mean of the ...
- 277
4
votes
Accepted
Can I use my computer while MinION is sequencing without negatively affecting the run?
Yes, that should be fine, especially if basecalling is disabled. Locking a screen should be fine as well.
I prefer to do basecalling after a run anyway, because there's a chance that it could chew up ...
- 13.8k
3
votes
Accepted
How many reads has my sequencing run produced on minion?
That's indeed the number of reads and that's quite low. How was your pore occupancy (number of pores sequencing) and your flow cell QC (number of pores good enough for sequencing)? How was your ...
- 1,314
3
votes
Accepted
Set directory where MinKNOW writes FAST5 files
I found a page explaining where this is set up in a user_conf file here:
https://community.nanoporetech.com/support/faq/test1/minknow/minknow/how-do-i-change-the-...
- 2,324
2
votes
Accepted
Does MinKNOW work with Mac OSX high sierra 10.13.1?
Some replies from ONT is:
OS X is supported and you can view the installation instructions on
our Downloads page linked below. At the moment High Sierra is not
officially supported as we have ...
- 2,459
1
vote
Can I stop my nanopore sequencing run if there are no more reads being produced?
Yes, you can stop the run and it should not affect the base calling. We normally do the runs and then do the base calling after the run has completed.
1
vote
Can I use my computer while MinION is sequencing without negatively affecting the run?
Since biological samples and reagents cost time and money, I wouldn't push it too much. Checking your email would be fine, but I wouldn't be browsing the web (besides gmail/calendar) in case a plug-...
- 106
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