2
votes
Accepted
How to convert Sars-CoV-2 ORF1ab codon positions to ORF1b positions?
ORF1ab:4588I corresponds to ORF1b:187I so one needs to subtract 4401 to go from ...
- 225
2
votes
Accepted
Bedtools get fasta and ORF from a blastX run
Blast uses a 1-based coordinate system. Bed is 0-based half open. Your code needs to convert appropriately. See for example: https://www.biostars.org/p/317112/ .
- 713
2
votes
Accepted
how to Know the longest ORF in Protein?
Proteins don't have ORFs, DNA sequences do. What DNA sequences do you have? If cDNA (i.e. transcript/mRNA) then an ORF finder will give multiple ORFs and the coordinates on the full-length input DNA ...
- 106
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