5
votes
Accepted
Estimate insert size for single-end data with picard CollectInsertSizeMetrics
CollectInsertSizeMetrics does not estimate but (as by the name) collects insert size metrics, which is nothing different than parsing the TLEN field from the BAM ...
2
votes
Accepted
Singularity & Picard
Assuming you've pulled the latest broadinstitute/picard container, your first attempt should have produced the expected result:
...
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