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5 votes

Estimate insert size for single-end data with picard CollectInsertSizeMetrics

CollectInsertSizeMetrics does not estimate but (as by the name) collects insert size metrics, which is nothing different than parsing the TLEN field from the BAM ...
ATpoint's user avatar
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2 votes

Singularity & Picard

Assuming you've pulled the latest broadinstitute/picard container, your first attempt should have produced the expected result: ...
Steve's user avatar
  • 3,059

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