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2 votes

Bar Graph of Expression Data from Seurat Object

Regarding the other answer with the below code: ...
Ruggero Belluomo's user avatar
0 votes

Can anyone share insights or provide the complete mRNA sequence for tdtomato-WPRE for scRNA seq analysis?

The Genbank sequence you've specified appears to be for RFP, not tdTomato. Have you tried one of the other tdTomato sequences, for example a lentiviral sequence, or a sequence that is called tdTomato? ...
gringer's user avatar
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2 votes

Can anyone share insights or provide the complete mRNA sequence for tdtomato-WPRE for scRNA seq analysis?

I'd suggest looking at the mouse itself, which appears to be here on the JAX website. This link includes RNA-seq experiments, so presumably you should be able to compare your pipeline and data to ...
Maximilian Press's user avatar

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