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4 votes

Error in quantile.default(x, p = p) in EgdeR calcNormFactors

Ok, it turned out that some rows and/or columns contained all values as zeros and that was the reason for the error. I wrote a function to fix that: ...
Nikita Vlasenko's user avatar
2 votes
Accepted

data visualization RNAseq : scaling data for PCA and cluster dendogram

Scaling (or centering) makes the genes comparable: Putting the expression levels of genes in the same scale (i.e. between 0 and 1) sustains that all of your genes contribute equally to the PCA or ...
haci's user avatar
  • 4,192
1 vote

Convert TPM-normalized matrices back to UMI in python

It depends what your downstream requirement is. If you want to do count based statisitcs, then you definately shouldn't just do TMM normalisation on the TPM data as count statistics only work on real ...
Ian Sudbery's user avatar
  • 3,331

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