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1 vote

Issues with adapter trimming (Trim Galore)

Looking at the plots, the only one I'd look into further is the per-sequence GC content, because the distribution is quite skewed in comparison to the theoretical distribution. This could be explained ...
gringer's user avatar
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2 votes

Issues with adapter trimming (Trim Galore)

Ignore the overrepresented sequences. The counts for these that you show is so low (10, 11) that it is irrelevant. Ignore per-base sequence content. This in RNA-seq is known to be a bit messed up ...
ATpoint's user avatar
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