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conchoecia
  • Member for 4 years, 5 months
  • Last seen more than a month ago
  • Santa Cruz, CA, United States
20 votes
8 answers
9k views

What is the fastest way to get the reverse complement of a DNA sequence in python?

13 votes
1 answer
714 views

What is the most compact data structure for canonical k-mers with the fastest lookup time?

10 votes
3 answers
7k views

What is the index fastq file (sample_I*.fastq.gz) generated when demultiplexing Illumina paired-end runs?

9 votes
3 answers
571 views

How to calculate the memory usage of storing kmers in RAM

8 votes
1 answer
2k views

How do kmer counters determine which kmer is 'canonical'?

7 votes
2 answers
716 views

How to write a hash function for canonical kmers?

7 votes
1 answer
692 views

Multithread fastq processing with kseq.h in C++11?

7 votes
2 answers
1k views

What is the correct way to map Hi-C data with bwa mem?

5 votes
1 answer
3k views

Why do NEBNext indexing primers have sequence between the p5 oligo and index?

4 votes
1 answer
512 views

How to map short sequences to long reads, recovering all multiply-mapped high-quality matches

4 votes
2 answers
355 views

How to align output of grep --color=always? (To QC fasta/fastq files)

4 votes
1 answer
64 views

Are there any Genome in a Bottle-like resources for non-humans (especially for invertebrates)?

3 votes
1 answer
371 views

How to generate two fasta files from samtools phase output?

3 votes
2 answers
192 views

How to map reads shorter than 32bp with minimap2?

2 votes
1 answer
1k views

Why are there more barcodes than GEMs in 10X chromium data?

2 votes
1 answer
153 views

How is consensus alignment for OLC assembly usually implemented?

2 votes
1 answer
274 views

How to force pilon to correct a reference with low coverage mapped reads?

2 votes
1 answer
175 views

Minimap2 -ax map-pb doesn't output tlen field

2 votes
1 answer
33 views

ENA minion 2D data doesn't actually have 2D data

2 votes
1 answer
393 views

Is nanopolish worth it since faster polishing software is available?

1 vote
1 answer
83 views

How to QC overamplified shotgun library?

1 vote
2 answers
2k views

Why do NEB adapters have non-complementary sequence?

1 vote
1 answer
105 views

Non-random access on a fastq file

1 vote
1 answer
1k views

What i5 index should I use on the Illumina sample sheet for an unindexed p5 primer?

0 votes
2 answers
56 views

Q: What analyses can I perform with a completely phased genome assembly?