I was wondering if there is a way to provide pacbio lima demultiplexing only a barcode, and let lima demux sample even if only one end has the barcode, not both?
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$\begingroup$ Can you please explain more about the context of your problem? What are you trying to do with the demultiplexing; what is the ultimate aim of the analysis? $\endgroup$– gringer ♦Commented Dec 21, 2023 at 9:08
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$\begingroup$ Some examples of what your data look like might help $\endgroup$– Maximilian PressCommented Dec 21, 2023 at 19:00
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PacBio lima need both 5'end and 3' end barcodes. You can have a unique barcode in one end and multiple on the other end; but it need two barcodes.
