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Regressing out unwanted sources of variation in single cell RNA-seq data
I have a dataset of single cell count data and I want to regress out the variation caused by the number of UMI's and the percentage of mitochondrial genes.
I know that count data is discrete data, ...
- 200
6
votes
1
answer
267
views
Scaling by linear regression against the number of reads
I am trying to build the preprocessing pipeline presented in The Tabula Muris Consortium et al. (pp).
It is a pipeline to preprocess single-cell sequencing data. There is one step that is not clear:
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