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Tagged with single-cell gene-expression
5 questions
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10X Cellranger count - Unable to distinguish between [SC5P-R2, SC3Pv2] chemistries based on the R2 read mapping for sample GEX in
Recently ran three separate single-cell experiments and sequenced them all using the 10X platform. Each of the experiments involved GEX, FBC and VDJ. With the resulting BCL files, csv files with info ...
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3
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How to split a Seurat cluster in several subclusters?
I've analyzed my scRNA-seq data and have a couple of Seurat clusters that show more than one cell type in each cluster. (for example, cluster 9 shows both NK and CD4 cells)
How can I split a cluster ...
- 21
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10X scRNAseq: Sample mix-up
The student who was working on scRNA seq of KO and WT lines has made a mistake and he mixed both lines and generate the final sequencing data. Now, we are having gene expression data but don't know ...
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5
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Where can I find Single Cell Data with Location "Coordinates"?
Does single cell data typically have the following meta-data: the "coordinates" (e.g. on a tissue, adjacent tissues) saying where each cell in the sample was located relative to other cells? ...
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Create heat map that groups genes by expression within cluster in Seurat
I have a set of cells that I am performing Drop-seq on to look at cell expression. Among my heat maps for gene expression I want to be able to graph them similar to the graph below:
Where the cells ...
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