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Pacific Biosciences is a biotech company selling a third-generation sequencing technology often denoted by the same name, also known as Single-Molecule Real-Time (SMRT) sequencing. Questions related to data generated by PacBio/SMRT sequencing.
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pacbio demultiplexing issue
I was wondering if there is a way to provide pacbio lima demultiplexing only a barcode, and let lima demux sample even if only one end has the barcode, not both? …
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Pacbio HIFI pbmm2 alignment metrics
I am new to pacbio sequencing data. I just did some alignment of pacbio HiFI data using pbmm2. I have the bam now, I would like to collect some metrics on the alignment. … I was wondering if there is similar tools for pacbio data. I searched and did not find an answer.
We are mainly interested in aligned rate, sequence length, coverage, accuracy, and GC content. …