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Results tagged with rna-seq
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user 224
Questions should include this tag if they pertain to issues related to bioinformatics analysis of RNA-seq data, e.g. normalization, differential expression analysis, sequencing and experimental design or transcriptome assembly...
2
votes
Importance of Proper Pairs vs Aligned Reads for RNASeq data
If you check the RSeQC docs you can get an explanation of how the inner distance stats work. By the look of your inner-distance MultiQC plot I guess that your sequencing is 2x100bp reads. This plot sh …
- 291
3
votes
5' and 3' bias in Rna-seq data
I know this is an old post, but if this plot is from after trimming I would suggest a different explanation: some trimming tools remove poly-A sequences from reads. If that's the case then any read en …
- 291