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Results tagged with differential-expression
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user 9042
Use it when comparing a measure of the expression between two (or more) samples, where the units are relative to the comparison.
2
votes
Comparing differential expression across samples - is batch effect correction needed?
There is no need for batch correction because all your comparisons are within batch. As long as you are making comparisons for each line, and treatment and control for the same line are in the same ba …
- 281
3
votes
Detecting differentially expressed genes with foldchange >= 2 and FDR < 0.05
The edgeR authors recommend that you use a relatively low logFC threshold for glmTreat such as lfc=log2(1.2). A lfc value as high as lfc=log2(2) is seldom required and only would only be appropriate f …
- 281
1
vote
Basic RNA Differential Expression in R
I'm thinking first normalize using TMM then compare the mean of the normalized values for treated vs. untreated?
That is a bit too simple. You need to analyse your experiment as a paired comparison. …
- 281
2
votes
Different results in differential expression analysis of microarray data
DE results do not change from one run to another. The code given in your question will give identical results each time you run it.
- 281