I would like to know the level of inbreeding for a single sample VCF file. I'm agnostic to the specific method of measuring inbreeding; any should work. However, I'd like to avoid having to specify a "background" or "reference" population. Ideally, the only input should be the single VCF file containing one human genome sample (WGS, hg38). (I will run this multiple times on different samples, but I'd like to run them individually.)
The existing tools seem to all require multiple VCFs.
Relatedly, is runs of homozygosity an acceptable metric for evaluating inbreeding in this context? And are there any special considerations to consider for various ethnic groups, like Ashkenazi Jews, that might influence the interpretation?