Questions tagged [best-practice]
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2 questions
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When performing differential expression analysis, should genes with low read counts be removed before or after normalization?
I have RNA seq data which I've quantified using Kallisto. I'd like to use tximport to transform the read count data into input for EdgeR, following the R code supplied in the tximport documentation:
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What are the ways to keep track of branches in the analysis?
I'm going through an RNA-seq pipeline in R/Bioconductor and want to try multiple parameters at subsequent steps, for example, running clustering with different ...
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