Questions tagged [qiime2]
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2 questions
2
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1
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How to adjust QIIME DADA2 parameters to avoid eliminating real sequences
I am working with sequence reads (acquired through MiSeq platform by Illumina) from a viral inoculum. I have already used FastP to merge paired-end reads and cut 5' and 3' ends to remove adapters and ...
3
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1
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30
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16S merging sequences, removes ALL reads
I am currently analysing some 16S (V4 region) rRNA data, using the qiime2 command-line software. Everything is working fine for the most part, until we are to merge the forward and reverse sequences - ...
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