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2 votes
1 answer
31 views

How to adjust QIIME DADA2 parameters to avoid eliminating real sequences

I am working with sequence reads (acquired through MiSeq platform by Illumina) from a viral inoculum. I have already used FastP to merge paired-end reads and cut 5' and 3' ends to remove adapters and ...
forlo_ov_hell's user avatar
3 votes
1 answer
30 views

16S merging sequences, removes ALL reads

I am currently analysing some 16S (V4 region) rRNA data, using the qiime2 command-line software. Everything is working fine for the most part, until we are to merge the forward and reverse sequences - ...
h3ab74's user avatar
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