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2 votes
2 answers
161 views

scRNA-seq differential transcript usage

Many of the modern gene-quantification tools (Salmon/Kallisto) output transcript-level (as opposed to gene-level) data. All of the scRNA-seq analysis I have seen just uses the gene-level values. I ...
burger's user avatar
  • 2,209
2 votes
1 answer
2k views

How to highlight specific cells in Seurat 2.4

I used Seurat 2.4 on our scRNA dataset to obtain the following tSNE plot.I was able to successfully extract cell IDs from the different clusters, and generate gene expression profiles. The analysis, ...
S K's user avatar
  • 21
2 votes
0 answers
202 views

pyScenic CLI for ctx is giving error: Not a single module loaded

Hi I ran pyScenic's ctx via the command in command prompt: ...
Yihua's user avatar
  • 21
1 vote
1 answer
44 views

How can to validate the presence of a certain type of cells in a single cell dataset?

I have a single cell dataset which I consider to be a reference , let say, for an human organ, I have identified some new clusters that correspond to cell types. I also have WGCNA modules that ...
MrPapouille's user avatar