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Subsetting of object existing of two samples
I have a Seurat object existing of an aggregate of two samples namely; RD1 and RD2. I am trying to make a subset of each sample. But it's not really working. I manage to make a subset of the cells but ...
- 131
2
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3
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Seurat: Error in FetchData.Seurat(object = object, vars = unique(x = expr.char[vars.use]), : None of the requested variables were found:
I was trying to do subsetting via:
pbmc = subset(pbmc, subset = nFeature > 1000 & nCount > 4000)
R returns:
...
- 21
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2
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Subsetting from seurat object based on orig.ident?
I am pretty new to Seurat. I want to subset from my original seurat object (BC3) meta.data based on orig.ident. however, when i use subset(), it returns with Error.
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