I've calculated the ANI of the streptomyces genus, but I'm confused about how to separate them into a different group, could anybody kindly tell me how to do that? Thanks!!!
ANI = average nucleotide identity.
This will give you a 2x2 matrix. You reconstruct a phylogeny using clustering, the most important being neighbor-joining, UPGMA is very occassionally used.
The statistic is clustering algorithm and its done by pairwise reconstruction of sister groups. Once a sister-group is defined (usually on nearest-neighor both in neighbor-joining and UPGMA) the genetic divergence from the hypothetical node is established either using the mean (UPGMA) or additive criteria (neighbor-joining). Okay enough theory ...
You get Mega X and find out the import format for a 2x2 distance matric. Mega X however will calculate this for you. You then go to the build tree menu heading and click "neighbor-joining" (or "distance"). The tree will very quickly automatically appear.