1. I used samtools depth to find the per base-pair read coverage over a number of isoform contigs from my Trinity assembly.
  2. I have also conducted a multiple sequence alignment of those isoforms using Clustal Omega DNA aligner.

In my final step, I want to assess the per-residue read coverage of the consensus amino acid sequence to demonstrate an idea of confidence in that sequence.

Does anyone have any tips on how to go about doing this and whether there are existing tools for such things?


1 Answer 1


Answer from @cephbirk, converted from comment:

What I ended up doing was to choose the read coverage for the lowest covered nucleotide in each codon as each residue's read coverage.


Your Answer

By clicking “Post Your Answer”, you agree to our terms of service and acknowledge you have read our privacy policy.

Not the answer you're looking for? Browse other questions tagged or ask your own question.