- I used
samtools depthto find the per base-pair read coverage over a number of isoform contigs from my Trinity assembly.
- I have also conducted a multiple sequence alignment of those isoforms using Clustal Omega DNA aligner.
In my final step, I want to assess the per-residue read coverage of the consensus amino acid sequence to demonstrate an idea of confidence in that sequence.
Does anyone have any tips on how to go about doing this and whether there are existing tools for such things?