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To be used for questions specific tothe sequence file format `.fasta`. Please minimise usage if the question is more generally about sequence formats.
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16S species-level taxonomic assignment--what is the current state of the art?
We currently use DADA2 for picking ASVs and the assignTaxonomy funciton for assingment to genera. Google does bring up various recent articles on species-level assignment with 16S.
Does anyone have …