I constructed a phylogenetic tree using a gene (example - secA). I had to gather the same gene sequence for all the required species from public database-NCBI and then constructed the tree after multiple sequence alignment. I used the MEGA X software.

I now want to constrict phylogenetic tree using multiple genes say 20. How do I go about it. I read about concatenating but I am not sure. Do I concatenate all gene sequences for each species and do a multiple alignment or perform an alignment for each gene and finally merge the alignments to get the tree? If possible, directions using MEGA X is helpful else any suggestions are welcome.


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