I constructed a phylogenetic tree using a gene (example - secA). I had to gather the same gene sequence for all the required species from public database-NCBI and then constructed the tree after multiple sequence alignment. I used the MEGA X software.
I now want to build a single phylogenetic tree using concatenated multiple genes, around 20.
How do I go about it. Do I,
- Concatenate all gene sequences for each species and do a multiple alignment or
- perform an alignment for each gene and finally concatenate the alignments to get the tree?
Could this be done using MEGA X?