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I constructed a phylogenetic tree using a gene (example - secA). I had to gather the same gene sequence for all the required species from public database-NCBI and then constructed the tree after multiple sequence alignment. I used the MEGA X software.

I now want to build a single phylogenetic tree using concatenated multiple genes, around 20.

How do I go about it. Do I,

  1. Concatenate all gene sequences for each species and do a multiple alignment or
  2. perform an alignment for each gene and finally concatenate the alignments to get the tree?

Could this be done using MEGA X?

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